Analyze, graph and present scientific data faster than ever with Prism 7. GraphPad Prism, available for both Windows and Mac computers, combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. GraphPad Prism was originally designed for experimental biologists in medical schools and drug companies, especially those in pharmacology and physiology. Prism is now used much more broadly by all kinds of biologists, as well as social and physical scientists. More than 200,000 scientists in over 110 countries rely on Prism to analyze, graph and present their scientific data. It is also widely used by undergraduate and graduate students. Nonlinear regression. Nonlinear regression is an important tool in analyzing data, but is often more difficult than it needs to be. No other program simplifies curve fitting like Prism. In fact, you can usually fit curves in a single step. Just select an equation from the extensive list of commonly used equations (or enter your own equation) and Prism does the rest automatically -- fits the curve, displays the results as a table, draws the curve on the graph, and interpolates unknown values. Place data for multiple data sets side-by-side on an organized data table, and Prism can fit them all the data sets at once. You can fit the same model separately to each data set, use global nonlinear regression to share parameter values among data sets, or fit different models to different data sets. Don't be fooled by the simplicity. Prism also gives you many advanced fitting options. It can report the confidence intervals of the best-fit parameters as asymmetrical ranges (profile likelihood method), which are far more accurate than the usual symmetrical intervals. It can also automatically interpolate unknown values from a standard curve (i.e. to analyze RIA data), compare the fits of two equations using an F test or Akaike's Information Criterion (AIC), plot residuals, identify outliers, differentially weight data points, test residuals for normality, and much more. Understandable statistics. While it won't replace a heavy-duty statistics program, Prism lets you easily perform basic statistical tests commonly used by laboratory and clinical researchers. Prism offers t tests, nonparametric comparisons, one-, two- and three-way ANOVA, analysis of contingency tables, and survival analysis. Analysis choices are presented in clear language that avoids unnecessary statistical jargon. Unlike other programs, Prism provides understandable statistical help when you need it. Press "Learn" from any data analysis dialog and Prism's online documentation will explain the principles of the analysis to help you make appropriate choices. Once you've made your choices, Prism presents the results on organized, easy-to-follow tables. The Prism documentation goes beyond anything you would expect. More than half of it is devoted to thorough explanations of basic statistics and nonlinear curve fitting, to teach you what you need to know to appropriately analyze your data. Analysis checklists. Once you’ve completed the analysis, Prism’s unique analysis checklists help you make sure you chose an analysis appropriate for your experimental design, and that you understand the assumptions behind the analysis. Retrace every analysis. It isn’t really science unless you can document exactly how your data were analyzed. WIth Prism this isn’t a problem. You’ll never wonder how the results got there. Even if the work was done by someone else, you can review (and change) all analysis choices, and see the sequence of analyses (i.e. that the X values were transformed to logarithms before the curve was fit). Similarly, you can easily check if error bars represent the SD or SEM (or something else. Automation without programming. All parts of your Prism project are linked. This means that when you fix a data entry error, Prism automatically updates all results, graphs, and layouts. Another advantage is that you can instantly analyze a repeat experiment. After you’ve polished the analysis and graphing steps with data from one experiment, you don’t have to repeat all those steps. Prism provides several ways to recycle your work -- to instantly analyze and graph a repeat experiment, without repeating any tedious steps and without requiring any scripting or programming. Prism also offers a scripting language for those who want more complex automation. Automatic error bars. If you enter raw data, Prism can automatically plot error bars as SD, SEM, range, interquartile range, or 95% confidence interval. If you computed error values elsewhere, enter them into labeled (SD or SEM) subcolumns. Prism’s analyses (i.e. t test, ANOVA, regression) will take into account the SD (or SEM) and sample size. A complete record of your work. A Prism project file can contain more than data, analyses and graphs. A Prism file can be a complete record of your experiments. Each file can contain any number of data tables, info pages, analysis results, graphs, and page layouts. Data tables can contain not only the data you analyze and graph, but also the data you decided to exclude, which remain on the data table (in blue italics). You can highlight values you want to review later. Each info page can store both unstructured notes and structured info constants such as lot numbers, concentrations, notebook page numbers, etc. Graphs and layouts can be embellished with text, lines, arrows, boxes, tables, equations, pictures and more. Annotate any page with color-coded floating notes, and highlight (in a color you choose) the names of pages you want to look at again, or want someone else to review. Save Prism files on your computer or network, or save directly to LabArchives. a cloud-based laboratory notebook. Ready to publish. Prism takes you from raw data to a graph (or layout) ready for presentations, posters or publications. A single click sends completed graphs or layouts to PowerPoint or Word. Copy and paste works too, of course. Export to TIFF, EPS, JPG, or PDF (and more), with all the options you need to satisfy the requirements of scientific journals. What's new in Prism 7. Prism 7 lets you do more kinds of statistical analyses, make more kinds of graphs, and organize your data with more flexibility. But Prism is still Prism! You can switch to Prism 7 with almost no learning curve, and can use the trial license of Prism 7 without removing earlier versions. Statistical comparisons. Paired or unpaired t tests. Reports P values and confidence intervals. Nonparametric Mann-Whitney test, including confidence interval of difference of medians. Kolmogorov-Smirnov test to compare two groups. Wilcoxon test with confidence interval of median. Perform many t tests at once, using False Discover Rate (or Bonferroni multiple comparisons) to choose which comparisons are discoveries to study further. Ordinary or repeated measures one-way ANOVA followed by the Tukey, Newman-Keuls, Dunnett, Bonferroni or Holm-Sidak multiple comparison tests, the post-test for trend, or Fisher’s Least Significant tests. Many multiple comparisons test are accompanied by confidence intervals and multiplicity adjusted P values. Greenhouse-Geisser correction so repeated measures one-way ANOVA does not have to assume sphericity. When this is chosen, multiple comparison tests also do not assume sphericity. Kruskal-Wallis or Friedman nonparametric one-way ANOVA with Dunn's post test. Fisher's exact test or the chi-square test. Calculate the relative risk and odds ratio with confidence intervals. Two-way ANOVA, even with missing values with some post tests. Two-way ANOVA, with repeated measures in one or both factors. Tukey, Newman-Keuls, Dunnett, Bonferron, Holm-Sidak, or Fishers LSD multiple comparisons testing main and simple effects. Three-way ANOVA (limited to two levels in two of the factors, and any number of levels in the third. Kaplan-Meier survival analysis. Compare curves with the log-rank test (including test for trend. Column statistics. Calculate min, max, quartiles, mean, SD, SEM, CI, CV. Mean or geometric mean with confidence intervals. Frequency distributions (bin to histogram), including cumulative histograms. Normality testing by three methods. One sample t test or Wilcoxon test to compare the column mean (or median) with a theoretical value. Skewness and Kurtosis. Identify outliers using Grubbs or ROUT method. Linear regression and correlation. Calculate slope and intercept with confidence intervals. Force the regression line through a specified point. Fit to replicate Y values or mean Y. Test for departure from linearity with a runs test. Calculate and graph residuals. Compare slopes and intercepts of two or more regression lines. Interpolate new points along the standard curve. Pearson or Spearman (nonparametric) correlation. Analyze a stack of P values, using Bonferroni multiple comparisons or the FDR approach to identify "significant" findings or discoveries. Nonlinear regression. Fit one of our 105 built-in equations, or enter your own. Enter differential or implicit equations. Enter different equations for different data sets. Global nonlinear regression – share parameters between data sets. Robust nonlinear regression. Automatic outlier identification or elimination. Compare models using extra sum-of-squares F test or AICc. Compare parameters between data sets. Apply constraints. Differentially weight points by several methods and assess how well your weighting method worked. Accept automatic initial estimated values or enter your own. Automatically graph curve over specified range of X values. Quantify precision of fits with SE or CI of parameters. Confidence intervals can be symmetrical (as is traditional) or asymmetrical (which is more accurate. Quantify symmetry of imprecision with Hougaard’s skewness. Plot confidence or prediction bands. Test normality of residuals. Runs or replicates test of adequacy of model. Report the covariance matrix or set of dependencies. Easily interpolate points from the best fit curve. Clinical (diagnostic) lab statistics. Bland-Altman plots. Receiver operator characteristic (ROC) curves. Deming regression (type ll linear regression. Simulate XY, Column or Contingency tables. Repeat analyses of simulated data as a Monte-Carlo analysis. Plot functions from equations you select or enter and parameter values you choose. Other calculations. Area under the curve, with confidence interval. Transform data. Identify outliers. Normality tests. Transpose tables. Subtract baseline (and combine columns. Compute each value as a fraction of its row, column or grand total. System requirements. Prism 7 for Windows runs under either the 32- or 64-bit versions of Windows Vista, 7, 8 or 10. Prism will run in a screen as small as 800 x 540. While it runs fine on 64 bit versions of Windows, it is a 32 bit program. Prism 7 for Macintosh requires OS X 10.9 (Mavericks) or later. If you use OS X 10.7 or 10.8, Prism will launch and seems to run ok but we haven't tested Prism thoroughly on these versions of OS X and can't provide much support. Prism requires 90 megabytes of space on the hard drive. Read what customers say about GraphPad Prism. On graphing. I was able to use the demo yesterday to create two layouts of publication-quality scientific graphics in an amazingly short time. I hope we're able to buy GraphPad Prism because this software is a pleasure to use, very intuitive and perfectly suited to my boss's needs and my abilities. Thank you for making such great software. I don't know how I could do my work without it. I have long been struggling to find the right program for graphing my data. Excel just would not do the trick and takes a lot of time to get used to it. I recently found Prism 5 for Mac OS and am reborn again. Every time I use your program I think it a joy that I have it. Its just easy to come back to and build great graphs even after long intervals away from it. On ease of use. I absolutely love your program. Ease of handling is unmatched and results are perfectly explained. Just what we non-computer geeks need. Prism has simplified my data analysis and taught me statistics at the same time! Thank you again for you help. I am very happy with the GraphPad program, you should be very proud. I am very dependent on it for analysis of vaccine immune responses, tumor survival, and just about everything else statistical. I'm a huge fan, and I'm glad to tell you how significant (yep, it was intentional) an impact you have had on my scientific life. This is the first time that I use this program. I am an undergraduate student and I have started a project on it. Your program has allowed me to analyze my data and create graphs faster and easier than any other program my school offers. Thanks so much for making my life 1,000,000,000x easier with your awesome program. On customer support. One of the many things I like about Prism is the speedy answer to my email questions. Thanks again. Your support is the best in the industry…thanks, you saved my weekend. …had no clue it was THAT easy, can't believe I've missed that a thousand times. Great guys and girls, you are so good in replying when one has questions. Thanks so much for your quick response. I really appreciate the detail you put into your response – what great support. I am impressed by your rapid response — Thanks a lot. Once again, you have shown me why yours is one of the best support systems. Thank you very much. Thanks, it is correct and I now understand how to change the Y axes. It would be great if PRISM could read my mind, I would marry it. In the meantime I am quite happy with what it can do, and I was able to “train” two other colleagues, by sharing what I have learned so far. They find it very useful as well. Thanks so much for your help! Thanks again for great support on a great product. Wow, a real person supporting a great product! I feel like I've gone back to the 80s or something. I'm consulting for a biotech firm. Their scientists are very happy with Prism, and I have passed-on to them the experience of working with you. Your extraordinary support, combined with the facility for scripting, project files, and many other features in Prism bode well for their continued use of your product! I was hired to automate some repetitive tasks for them. Your excellent help files, especially the scripting examples, really made me look good! Thanks. Even though I am not a programmer, I do understand that bugs will always persist in very complex code. What puts GraphPad in a separate category from other scientific software developers/vendors is that even the slightest problems that will ultimately improve the end-user experience get fixed or revised (I can attest for this). I have used several other scientific software packages and I always try to report bugs or usability issues. Unlike my experience with GraphPad, I rarely I get a response from other companies, and I often see the same bugs being carried over from the current version to the next release. On statistics. Many congratulations for Prism. I was going to say that it's one of the best software I've used as a molecular biologist, but actually, it's THE best. Finally a statistics program in English (and not in "statistician"). Well done. I would like to express my enthusiasm for GraphPad Prism which I have used for the last 2-3 years. Now on ver. 5 it has become an invaluable statistical tool in my research, and I always try to share my delight for this software with colleagues not introduced to GP. The fact that you have made statistics (more) easy to understand for an non-statistician is really an accomplishment. I would like to take the opportunity to congratulate you for your fantastic program. I've worked with several other statistics programs, and, as a non-statistician, really enjoy working with Prism because it is so much easier and much more intuitive than other programs. The excellent documentation and statistics guide was really helpful during my first statistical steps. So…no more !#%$^%$% Excel for analyzing scientific data! I've become a Prism evangelist. It blows the other statistical programs out of the water! I would be lost without Prism. Prism is a beautiful piece of software. I use it constantly to figure out biological mysteries. Just want to congratulate you for creating this very intuitive, user friendly and flexible software. Now I just need to convince my department to buy me one. This program is excellent for me. Prism is the best I have tried because it is flexible and simple for use and evaluating functions when modeling. Thank you so much! This was very helpful. Prism is a really great program, and very user-friendly for us non-math geniuses. Thanks again for all of your help, you have gone far and beyond what I was expecting. You make a great point, that I do not have independent observations. That is something that a manuscript reviewer may have picked up on, but thanks to you I will have that covered. You have shown me that you are committed fully to your company and with this level of ‘support' I would give your product my highest level of recommendation. Aside from all of the above I must tell you that I am an Origin user and have now used a trial version of your Prism for MAC and must say that I must have called my wife over ten times to witness the marvels of your package on my MAC screen. The ease of graphing and changing the graphic appearance automatically is remarkable. I just started the trial. I just discovered the automatic color choices gallery as icing on the cake. Your software motivated me to try to tackle statistical principles one last time, that's why I ordered your book. Many thanks. It's a great program. I've tried to encourage MacUser to mention it when they're discussing graph-drawing, because so many packages are just business-orientated (no provision for error bars on graphs, etc.) but not too successful, I'm afraid. Best wishes and thanks again for your prompt help. Thanks for your reply, I've been doing what you've suggested. I really enjoy your software, it's incredible. We couldn't do without your product. It is the best. Thank you so much. I love your software and it is great for non statisticians. Thank you. I would be lost without Prism. I'm a huge fan, and I'm glad to tell you how significant (yep, it was intentional) an impact you have had on my scientific life. Prism has incredible sophistication and complexity that the user never senses because the interface is so superbly designed. Thank you for a tremendous tool. It would be great if PRISM could read my mind, I would marry it. I must have called my wife over ten times to witness the marvels of your package on my MAC screen. Every time I use your program I think it a joy that I have it. On Help files. I congratulate you on making such a great program because it's so user-friendly and the explanations given on your site about the tests etc. are better than any text-book I've read thus far in my studies. Brilliant software, with very useful Help. I love the program and I find it to be an excellent resource for not only statistical analysis, but also an invaluable tool for understanding and applying correct analyses for my research data. I find the Prism help manuals to be of textbook quality, certainly far better than any other end user manual I'm familiar with. This is due to the fact that they teach you how to use the program, and also they teach you a lot of the background subject matter. Just a quick one to thank you for the great product you offer to answer our scientific plotting needs and the continuously accurate and efficient technical help you provide, you and your team. Just finished reviewing some of my grads data, and thought that rarely do we take the time to tell about our appreciation of a good product. One of my PhD students, again this week, told me how "Prism help" makes him discover and appreciate biostatistics every day. Unfortunately, basic statistics courses are optional in our medicine faculty (an issue I'm working on resolving soon I hope). Meanwhile, we often refer to some of Prism's clear content to discuss about stats in the lab. Prism has the only Help screens worth reading as they are an actual treatise on data analysis. Compared to other software. I tried GraphPad [Prism] based on the recommendation of a colleague at another University and loved it! It blows the other statistical programs out of the water for the construction of clear and attractive tables, graphs and figures for publication. I have to tell you that Prism is one my favorite programs of all time and this is from someone who has been fitting functions to response curves for 30 years, dating back to punch card days on the campus IBM mainframe during graduate school. I switched to the PC version of SAS before your program came out, but when a colleague showed me Prism at Duke in the early mid 90's (I think), SAS was toast and I've never looked back. I flirted briefly with another program (Slidewrite) during those days, but it lacked to analytical power of your program. Thank you. I would have taken ages to figure that out. GraphPad's support is the BEST. My lab just purchased 4 individual licenses for Prism 5 this week. I'd looked into SAS as well, but went with Prism because it was so easy to install the 30-day demo and actually see for myself how “intuitive” it is to use— and how helpful the tutorials, help files, etc actually are. So…no more !#%$^%$% Excel for analyzing scientific data! I've become a Prism evangelist. Hello, Thank you so much! This is exactly what we needed. You have saved us a ton of time in re-doing our calculations in Excel. While the rest of my lab uses an assortment of graphing and statistical analysis software (Sigma Plot, SAS, Excel), I strongly prefer Prism. On a colleague's recommendation, I downloaded the trial version, and it has been unbelievably helpful. As a matter of fact, because Prism is so user-friendly, intuitive, and informative, I now am much more confident in performing statistical analyses, whereas before, I was always intimidated by statistics and unsure of what I was doing. Online Prism Guides. Our online help goes way beyond what you'd expect. It doesn't just teach you to use Prism, but also helps you learn about data analysis and curve fitting. Help within Prism. Tutorial data sets. When you create a new table from the Welcome dialog, you can create an empty table or choose a tutorial data set. Each of these comes with data, a brief explanation, and a link to a more comprehensive explanation in the Prism online help. Graph Portfolio. Choose this tab in the Welcome dialog, and browse dozens of polished graphs. Open any one to see how it was made. Many come with linked explanations. Step by step tutorials. Statistical tutorials. T test, contingency tables, survival curves and more. Curve fitting. Enzyme kinetics, exponential decay with outlier detection, dose-response with global fitting, and more. YouTube videos. Several people have created videos to introduce you to Prism. Check them out.

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